Fig. 2

MST4 deficiency enhances the activation of IRF3. A, B Luciferase activity of lysates of 293 T-sh-Control and 293 T-sh-MST4 cells transfected with either ISRE-Luc (A) or NF-κB-Luc (B), plus β-gal for 24 h, followed by the infection with SeV or transfection with Poly (I:C) for 8 h. C Immunoblot analysis of the indicated proteins in 293 T-sh-Control and 293 T-sh-MST4 cells infected with SeV at the indicated time points. D Immunoblot analysis of the indicated proteins in 293 T-sh-Control and 293 T-sh-MST4 cells infected with SeV for 8 h, followed by the nuclear-cytoplasm extraction. E Immunoblot analysis of the indicated proteins in 293 T cells transfected with Myc-MST4 or control vector treated with SeV for 8 h, followed by the nuclear-cytoplasm extraction. F Confocal microscopy of endogenous IRF3 in 293 T-sh-Control and 293 T-sh-MST4 cells infected with SeV for 8 h. Scale bars, 25 µm. Data information: The data are represented as mean ± SD (A, B: n = 3). **P < 0.01 (unpaired two-tailed Student’s t-test). The data are representative of at least three independent experiments