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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: NEK2 promotes the migration and proliferation of ESCC via stabilization of YAP1 by phosphorylation at Thr-143

Fig. 2

Knockdown of NEK2 leads to the impairment of migration and proliferation of ESCC in vitro. A The RNA-seq data of CCLE showed various expressions of NEK2 in ESCC. B The expression of NEK2 in ESCC was significantly higher than immortalized esophageal epithelia Het-1A in transcriptional and translational levels. C Both shRNA-2 and shRNA-3 in KYSE-510, as well as all strings in Eca-109, yielded efficacy in knocking down NEK2 at the translational level. D Knockdown of NEK2 in both cells showed declined migration in Transwell assay. E Knockdown of NEK2 in both cells exhibits decreased proliferation in clone formation assay. All experiments were carried out independently for three times. *P < 0.05, **P < 0.01, ***P < 0.001. CCLE Cancer Cell Line Encyclopedia

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Cell Communication and Signaling

ISSN: 1478-811X