Fig. 7
From: Inhibition of AKT induces p53/SIRT6/PARP1-dependent parthanatos to suppress tumor growth
ROS promotes AKT inhibition induced parthanatos. a ROS production in HCT116 was tested by fluorescence microscope after different treatments for 12hm. b ROS positive cells rates after treatment. c Cell viability of HCT116 was detected by CCK-8 assay after administrated 10 μM SC66 and 10 μM TIC10 for 24 h with or without 20 μM NAC. d The expressions of PAR polymer and PARP1 in HCT116 were tested after administrated 10 μM SC66 and 10 μM TIC10 for 24 h with or without 20 μM NAC by western blotting assay. e Immunofluorescent analysis of AIF (green) translocation under SC66 (10 μM) treatment with or without NAC (10 μM). f Immunofluorescent stain for the PAR polymer (red). **p < 0.01, ****p < 0.0001