Fig. 2
From: Inhibition of AKT induces p53/SIRT6/PARP1-dependent parthanatos to suppress tumor growth
AKT inhibition triggers parthanatos and autophagy. a, b Cell viability of HCT116 was detected by CCK-8 assay after administrated 2 μM SC66 (a) and 10 μM TIC10 (b) for 48 h with or without 10 μM Z-VAD (caspase inhibitor). c The expressions of cleaved-caspase1, GPX4, p-MLKL (S358), PARP1, cleaved PARP1 and PAR in HCT116 were tested after administrated 10 μM SC66 with or without Z-VAD. d Western blotting assay tested the level of LC3, p62 and PAR in HCT116 treated with SC66 or TIC10. e Western blotting assay tested the level of AKT, LC3 and PAR in HCT116 after knock down of AKT. f, g, h Qualitative analysis of LC3, p62 and PAR levels under SC66 (f), TIC10 (g) and AKT knock down (h). i Immunofluorescent analysis of the PAR polymer (red) accumulation in HCT116 with knock down of AKT