Fig. 1

AKT inhibition promoted cell death in colorectal cancer cells. a, b Cell viability of HCT116 was analyzed by CCK-8 assay after administration of SC66 (0.33 μM, 1 μM, 3.3 μM, 10 μM and 33 μM) or TIC10 (1 μM, 3.3 μM, 10 μM, 33 μM and 100 μM) at the time points of 24 h, 48 h and 72 h. c Colony formation assay by crystal violet staining in HCT116 treated with SC66 (2 μM, 10 μM) and TIC10 (5 μM, 10 μM) after 14 days. d Hoechst 33,258 staining was performed after 10 μM of SC66 treatment for 24 h e After HCT116 cells were treated with 10 μM of SC66 and TIC10 for 24 h and 48 h respectively, the level of total-caspase3 and cleaved-caspase3 was tested via western blotting. f The expression levels of p-Akt (S473), p-FoxO3a (S253) and p-p65(S276) were detected after the treatment of SC66 (2 μM, 10 μM) for 24 h and TIC10 (10 μM) for 24 h, 48 h or 72 h in HCT116