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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Tumor-associated macrophages promote epithelial–mesenchymal transition and the cancer stem cell properties in triple-negative breast cancer through CCL2/AKT/β-catenin signaling

Fig. 5

TAMs activate β-catenin signaling by CCL2/AKT signaling. A qRT-PCR analysis of CCL2 mRNA levels in M0 and M2 macrophages. B ELISA analysis of CCL2 secretion in M0 and M2 macrophages. C Western blotting analysis for total β-catenin and phospho-β-catenin (S552) levels in BT549 and HCC1937 cells induced by CCL2 at different concentrations. D Western blotting showing nuclear and cytoplasmic protein levels of total β-catenin in BT549 and HCC1937 cells induced by CCL2. E Western blotting analysis for total β-catenin and phospho-β-catenin (S552) levels in BT549 and HCC1937 cells cultured with M0-CM or M2-CM followed by treated with CCR2 inhibitor. F, G Western blotting showing nuclear and cytoplasmic protein levels of total β-catenin in BT549 and HCC1937 cells cultured with M0-CM or M2-CM followed by treated with CCR2 inhibitor. H Transwell assays showing the invasive ability of BT549 and HCC1937 cells cultured with M0-CM or M2-CM followed by treated with CCR2 inhibitor. Scale bar = 100 μm. I Wound healing assays showing the migratory ability of BT549 and HCC1937 cells cultured with M0-CM or M2-CM followed by treated with CCR2 inhibitor. Scale bar = 100 μm. J Western blotting analysis of the protein levels of p-JNK, p-ERK2 and p-AKT in BT549 and HCC1937 cells cultured with M0-CM or M2-CM followed by treated with CCR2 inhibitor. K Western blotting analysis for total β-catenin and phospho-β-catenin (S552) levels in BT549 and HCC1937 cells cultured with M0-CM or M2-CM followed by treated with AKT inhibitor LY294002. N = 3. *p < 0.05. **p < 0.01

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Cell Communication and Signaling

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