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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Tumor-associated macrophages promote epithelial–mesenchymal transition and the cancer stem cell properties in triple-negative breast cancer through CCL2/AKT/β-catenin signaling

Fig. 3

TAMs promote cancer stem cell properties in BT549 and HCC1937 cells. A Flow cytometry analysis for the CD44+/CD24 cell populations in BT549 and HCC1937 cells cultured with M0-CM or M2-CM. B Flow cytometry analysis for the ALDH+ subpopulations in BT549 cells cultured with M0-CM or M2-CM. C qRT-PCR analysis of SOX2, OCT4, and Nanog mRNA levels in BT549 and HCC1937 cells cultured with M0-CM or M2-CM. D Western blotting analysis for SOX2, OCT4, and Nanog expression in BT549 and HCC1937 cells cultured with M0-CM or M2-CM. N = 3. *p < 0.05. **p < 0.01

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