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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Optogenetic control of NOTCH1 signaling

Fig. 3

The total size of breast cancer cell spheroids. The LOV2V416L-Zdk1-N1ICD (oN) and wild type (WT) MCF7 and MDA-MB-468 cells were cultivated in Matrigel with 10 µM DAPT in duplicated 96-well plates. a MDA-MB-468 and c MCF7 cells expressing LOV2V416L-Zdk1-N1ICD (oN) and wild type (WT) cells (b, d, respectively) are shown. Light (red line)—cells light-activated for 3 h every day; Dark (black lines)—not light stimulated cells. eh representative graphs of stable LOV2V416L-Zdk1-N1ICD and WT breast cancer spheroids were taken every 48 h for the duration of the experiment (8 days). The spheroids (n = 7) area was analyzed every 48 h for 8 days. Area of spheroids MDA-MB-468 (i) and MCF7 (j), oN and WT, obtained through sandwich assay, which is characterized by the growth of spheroids from single cells. Spheroids were cultured between two layers of Matrigel (Lower gel − 4 mg/ml and Upper gel − 2 mg/ml) with 10 µM DAPT and controls without this inhibitor. Each spheroid area was analyzed every 72 h for 12 days through AMIDA software. Light (red line)—cells light-activated for 3 h every day; Dark (black lines)—not light stimulated cells. Comparison of the morphology of spheroids formed by oN MDA-MB-468 (k) and oN MCF7 (l) photo-stimulated (LIGHT) or kept in the dark (DARK)

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