Fig. 9

p38/MAPK activation mediated HG-induced podocyte apoptosis and cytoskeleton. A, B The permeability of podocyte was assessed by measuring the influx of FITC-conjugated dextran and the TER across the cells. C, D TUNEL staining showed podocyte apoptosis. Bar, 25 µm. E Representative confocal immunofluorescence images showing the MTs and F-actin organization of the podocytes. Bar, 10 μm. The inserts showed high magnification images of the MT and F-actin network. F Representative immunofluorescence images showing the MTs and F-actin organization of the podocyte after the cells were transiently transfected with MAP4(Ala), MKK6(Glu) or both. Bar, 10 μm. The inserts showed high magnification images of the peripheral MT network. Data were shown as mean ± SEM. The experiment was conducted 6 times. *P < 0.05, **P < 0.01, ***P < 0.001. P values were derived from one-way ANOVA with Bonferroni’s post-test