Fig. 2

Nephritic MAP4 hyperphosphorylation along with proteinuria in DN. A, B Body weight (A) and random blood glucose (B) was assessed between WT and WT STZ mice. n = 8. C, D Representative WB (C) and quantitative analysis (D) depicting p-M (S737 and S760) in WT and WT STZ littermates. n = 6. p-M, p-MAP4. E–H UACR, serum levels of CR, UN and Cys-c were detected by commercial kits. n = 8. I, J HE staining and glomerular volume quantitative analysis of deparaffinized kidney tissue section between WT and WT STZ mice. Bar, 20 µm. n = 6. K Masson’s trichrome staining of WT and WT STZ mice. Bar, 20 µm. n = 6. L, M Quantitative analysis of foot processes fusion (L) and TEM observation (M). Bar, 5 µm. n = 6. N, O Representative confocal immunofluorescence images and quantitative analysis showing the epithelial and mesenchymal cell markers of frozen kidney tissue section. Bar, 10 or 25 µm. n = 6. P, Q Podocyte apoptosis (white arrows) of frozen kidney tissue section was detected by WT-1 and TUNEL co-staining. Bar, 10 µm. n = 6. R, S Cell proliferation (white arrows) of frozen kidney tissue section was determined by EdU staining. Bar, 10 µm. n = 6. The graph showed mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. P values were derived from two-tailed Student's t-test