Fig. 6

The suppression of ezrin inhibited the LPS-induced production of cytokines. A549 cells were pre-treated with Y-27632 and subjected to LPS. The release of TNF-α, IL-1β, and HMGB1 into the supernatants was then measured by ELISA (a–c). The expression level of HMGB1 in cell lysates was measured by western blotting (d). Cell viability was examined by CCK-8 assay (e). A549 cells were transfected with ezrin-specific siRNA; TNF-α, and IL-1β expression was measured by ELISA (f–h), and the cellular levels of HMGB1 was measured by western blotting (i). Data are expressed as mean ± SD of triplicate samples. *p < 0.05 versus control group, #p < 0.05 versus LPS group