Fig. 5

The suppression of ezrin inhibited LPS-induced activation of p38 and NF-κB. A549 cells were transfected with ezrin siRNA or control siRNA. The expression of ezrin mRNA was detected by qRT-PCR (a) and the protein level of ezrin was measured by western blotting (b). The effect of ezrin siRNA transfection on IKK, IκBα, and MAPKs activation was detected by western blotting (c–h), and NF-κB activation was determined by EMSA (i). Data are expressed as mean ± SD of triplicate samples. *p < 0.05 versus LPS(-) control siRNA group, #p < 0.05 versus LPS(+) control siRNA group