Skip to main content
Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Epigenetic silencing and tumor suppressor gene of HAND2 by targeting ERK signaling in colorectal cancer

Fig. 5

HAND2 could regulate MAPK/ERK signaling pathway by reducing phosphorylation of ERK. a Deep RNA sequencing were conducted to compare the mRNA expression profile between DLD1 cells with HAND2 reconstitute cells (HAND2-OE) and control vector. (Control) (n = 3). Thousands of aberrant expressed genes were identified in heatmap analysis. b, c Both PTEN and KRAS pathways were upregulated (b) while ERK signaling were downregulated (c) after HAND2 reconstitution by GSEA analysis of RNA-seq data. d Decreased P-ERK were observed in HAND2 overexpressed cells (HAND2OE), while no significant changes of EGFR, RAS, PTEN and ERK were shown in both DLD1 and RKO cells by western blot. e HAND2 could directly bind to ERK in both cells with HAND2 constitution (DLD1-PCDH-HAND2OE) and wild type CRC cells (HCT8WT), while the binding capacity with p-ERK was greatly decreased by CoIP assays

Back to article page