Fig. 2

Roles of TRIM15 in promoting NSCLC growth and metastasis. A Expression levels of TRIM15 in the indicated lung cancer cell lines were analysed by western blotting. Confirmation of TRIM15 knockdown in H1299. H1650 cells were transfected with control plasmids or vector expressing TRIM15 or TRIM15-ΔRING. Immunoblot detection of TRIM15 and TRIM15-ΔRING expression in H1650 cells. B, C The effect of TRIM15 knockdown and overexpression on NSCLC cells proliferation was assessed by CCK8 and EdU-incorporation assay. D, E The effects of TRIM15 loss- or gain-of-function on migration (D) and invasion (E) of NSCLC cells. Knockdown of TRIM15 resulted in significant inhibited migration (D) and invasion (E) of H1299 cells. TRIM15 upregulation significantly increased cancer cell migration (D) and invasion (E), while the TRIM15-ΔRING lost this ability. Statistical analyses were performed by two-tailed unpaired Student’s t-test. **P < 0.01