Fig. 4From: CCL7 playing a dominant role in recruiting early OCPs to facilitate osteolysis at metastatic site of colorectal cancerLactate upregulated expression of c-Jun through activating JNK pathway. A qRT-PCR analysis detected mRNA level of CCL7 in early OCPs after transfection with c-Fos siRNA or c-Jun siRNA. n = 3. B qRT-PCR analysis detected mRNA level of CCL7 in early OCPs after treatment of lactate in the presence of MC-38 CM or not. n = 3. C–F Western blotting analysis showed protein levels of c-Jun, JNK, p38 MAPK and their total protein in early OCPs after stimulated with lactate in the presence of MC-38 CM or not (B) and the quantification of protein level normalized to β-actin (C–E). n = 3. G, H Western blotting analysis showed protein levels of c-Jun in early OCPs after stimulated with lactate plus p38 MAPK antagonist or JNK antagonist in the presence of MC-38 CM (F) and the quantification of protein level normalized to β-actin (G). n = 3. *p < 0.05, **p < 0.01, ***p < 0.001Back to article page