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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Oligodendroglia-derived extracellular vesicles activate autophagy via LC3B/BAG3 to protect against oxidative stress with an enhanced effect for HSPB8 enriched vesicles

Fig. 1

Characterization of human oligodendroglia-derived extracellular vesicles. A Western blotting analysis of small extracellular vesicle protein markers CD63, CD9, Flotillin-1, Annexin A2, HSP70, and the negative control marker GRP94. B Representative overview (scale bar 200 nm) and cropped (scale bar 100 nm) image showing the morphology, as revealed by transmission electron microscopy, of EVs isolated from wild-type native human oligodendroglia (OL-EVs) and stably expressing HSPB8 oligodendroglia (OL-HSPB8-EV). C–E Particle size profile distribution, particles per mL, and total EV protein measured by Nanoparticle Tracking Analysis (NTA) and BCA of OL-EVs and OL-HSPB8-EVs, pelleted from 30 mL of conditioned medium. F–H Western blotting and qPCR analysis of HSPB8 present in OL-EVs and OL-HSPB8-EVs. Statistical analysis was performed using GraphPad. Student’s t-test was used to determine significance (**P < 0.01, ****P < 0.0001). Data are presented as mean ± SEM

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Cell Communication and Signaling

ISSN: 1478-811X