Fig. 7

si-CACNA1d inhibited the proliferation of GC and could be rescued by tRF-Val-CAC-016 inhibitor. a–b In the CCK-8 assays, si-CACNA1d could significantly decline the proliferation of GC cells and was partly recovered by tRF-Val-CAC-016 inhibitor in HGC-27 and NCI-N87. c–d In the EdU assays, the tRF-Val-CAC-016 inhibitor could rescue the inhibitory effect of si-CACNA1d in terms of cell replication activity. e–g si-CACNA1d resulted in the G1 phase arrest in HGC-27, but S phase arrest in NCI-N87 with flow cytometry and could both be rescued by tRF-Val-CAC-016 inhibitor. h–i The rescue effect of the tRF-Val-CAC-016 inhibitor on si-CACNA1d was also confirmed in the colony formation assays. j–k The suppressive function of si-CACNA1d on the protein expression of CACNA1d, CyclinD1, CyclinB, c-myc was partly resumed by tRF-Val-CAC-016 inhibitor. *P < 0.05, **P < 0.01, statistically significant