Fig. 2

tRF-Val-CAC-016 was significantly low-expressed in GC tissues. a–b Gel-electrophoresis was applied to verify the PCR product of tRF-Val-CAC-016, and the Sanger sequencing was undertaken to confirm the results. c Fluorescent In Situ Hybridization (FISH) assay indicated that tRF-Val-CAC-016 is located in both nuclei and cytoplasm, and mainly in the cytoplasm. d The expression levels of tRF-Val-CAC-016 were tested in GC cell lines, NCI-N87 and HGC-27 were finally selected. e tRF-Val-CAC-016 mimics could significantly increase the expression level of tRF-Val-CAC-016. f The low expression level of tRF-Val-CAC-016 was confirmed in 40 pairs of GC tissues. g Correlation between the expression level of tRF-Val-CAC-016 and the long-term outcomes (overall survival, OS) of GC was not significant (P = 0.5802). *P < 0.05, **P < 0.01, statistically significant. Scale bar = 10 µm