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Fig. 11 | Cell Communication and Signaling

Fig. 11

From: tRF-Val-CAC-016 modulates the transduction of CACNA1d-mediated MAPK signaling pathways to suppress the proliferation of gastric carcinoma

Fig. 11

tRNA derivative tRF-Val-CAC-016 modulates the canonical MAPK signaling pathway by targeting CACNA1d. a The mechanism diagram clearly explained the pattern that tRF-Val-CAC-016 exerted its influence on the MAPK signaling pathway. b In the immunoblotting assays of NCI-N87, tRF-Val-CAC-016 mimics suppressed the expression of CACNA1d (Cav1.3), ERK, p-ERK, and p-p38. tRF-Val-CAC-016 inhibitor enhanced the expression of CACNA1d (Cav1.3), ERK, p-JNK, p38, cyclinB, cyclinD1, c-myc. c si-CACNA1d could inhibit the expression levels of CACNA1d (Cav1.3), JNK, p-p38, cyclinD1, and c-myc, which was able to be reversed by tRF-Val-CAC-016 inhibitor. d pcDNA-CACNA1d increased the expression of CACNA1d (Cav1.3), ERK, p-ERK, p-JNK, p38, p-p38, cyclinB, cyclinD1, and c-myc, which was rescued by tRF-Val-CAC-016 mimics. e In HGC-27, tRF-Val-CAC-016 mimics declined, but tRF-Val-CAC-016 inhibitor promoted the protein levels of CACNA1d (Cav1.3), ERK, p-ERK, JNK, p-JNK, p38, p-p38, cyclinB, cyclinD1, c-myc. f CACNA1d (Cav1.3), ERK, JNK, p-JNK, p38, p-p38, cyclinB, and cyclinD1 were down-regulated by si-CACNA1d, and this effect was reversed by tRF-Val-CAC-016 inhibitor. g CACNA1d (Cav1.3), ERK, p-ERK, JNK, p-JNK, p38, p-p38, cyclinB, cyclinD1, and c-myc were enhanced by pcDNA-CACNA1d, which could be deteriorated by tRF-Val-CAC-016 mimics

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