Fig. 1

Nutlin-3 elevated the sensitivity of MCF-7 cells to THZ1. A Breast cancer cells were treated with increasing concentrations of THZ1 for 24 h and detected their cell viabilities by CCK8 assay. The IC50 values were calculated using GraphPad Prism software. B The cell viabilities of various breast cancer cells after treating with 50 nM THZ1 for 24 h and 48 h respectively were detected by CCK8 assay. C The colony formation ability was detected after breast cancer cells were treated with 50 nM THZ1. D Quantitation of colony formation ability in C. E, F The cell viability was detected by CCK8 assay after MCF-7 cell was treated with increasing concentrations of nutlin-3 or THZ1 for 24 h or 48 h separately. G The cell viability was detected by CCK8 assay after MCF-7 cell was treated with 10 nM or 20 nM nutlin-3 combining with 50 nM THZ1 for 24 h, 48 h or 72 h separately. H The colony formation ability was detected after MCF-7 cell was treated with 10 nM or 20 nM nutlin-3 combining with 50 nM THZ1. I Quantitation of colony formation ability in H. J Apoptosis was analyzed by flow cytometry after MCF-7 cell was treated with 10 nM or 20 nM nutlin-3 combining with 50 nM THZ1 for 48 h. K Quantity of apoptosis in J. L The protein levels of cleaved PARP and p53 were detected by western blot. M Quantity of cleaved PARP and P53 severally in L. Statistically significant (P < 0.05) are *, (P < 0.01) are **