Skip to main content
Fig. 7 | Cell Communication and Signaling

Fig. 7

From: Sec23a inhibits the self-renewal of melanoma cancer stem cells via inactivation of ER-phagy

Fig. 7

Sec23a inhibits the stemness of A375-SE cells via inactivation of ER stress and autophagy. a Analysis of mRNA expression of Aldh1, CD133, Nanog, Oct4 and Sox2. GAPDH expression was used as a reference control. b Assessment of the clonogenic spheroid formation rate of A375-Parental and A375-SE cells measured by the single-cell cloning assay. c Tumors resected from nude mice inoculated with A375-Parental and A375-SE cells, respectively, bar = 1 cm. d Tumor weight of nude mice to which A375-Parental and A375-SE cells were injected respectively. e RT-qPCR was performed to measure Sec23a expression in A375-Parental and A375-SE cells. f RT-qPCR was performed to confirm gene manipulation of Sec23a expression in A375-SE cells. g Analysis of mRNA expression of Aldh1, CD133, Nanog, Oct4 and Sox2 in A375-SE cells with Sec23a interference or overexpression. GAPDH expression was used as a reference control. h Assessment of the clonogenic spheroid formation rate of A375-SE cells with Sec23a interference or overexpression by the single-cell cloning assay. i Western blot analysis for LC3I/II and P62 in A375-Parental and A375-SE cells. j Western blot analysis for LC3I/II and P62 in A375-SE cells with Sec23a interference or overexpression. k Analysis of mRNA expression of ER stress marker genes in A375-SE cells with Sec23a interference or overexpression. l RT-qPCR was performed to confirm FAM134B interference in A375-SE-shSec23a cells. m Western blot analysis for LC3I/II and P62 in A375-SE-shSec23a cells with FAM134B interference. n Analysis of mRNA expression of Aldh1, CD133, Nanog, Oct4 and Sox2 in A375-SE-shSec23a cells with FAM134B interference. o Assessment of the clonogenic spheroid formation rate of A375-SE-shSec23a cells with FAM134B interference by the single-cell cloning assay. *P < 0.05, **P < 0.01, ***P < 0.001

Back to article page