Fig. 5
From: Sec23a inhibits the self-renewal of melanoma cancer stem cells via inactivation of ER-phagy
Sec23a inhibits autophagy in M14-SE cells via inhibition of ER stress. a Analysis of mRNA expression of ER stress marker genes in M14-SE cells with Sec23a interference or overexpression. b The morphology of ER in M14-SE cells with Sec23a interference or overexpression under transmission electron microscopy, bar = 5 μm. c and d RT-qPCR and Western blot were performed to measure FAM134B expression in M14-SE cells with Sec23a interference or overexpression. e RT-qPCR was performed to confirm effective FAM134B interference in M14-SE-shSec23a cells. f Western blot analysis for FAM134B, LC3I/II and P62 in M14-SE-shSec23a cells upon FAM134B interference. g Representatives of LC3B-positive puncta images were shown in M14-SE-shSec23a cells upon FAM134B interference, bar = 20 μm. h Transmission electron microscopy of M14-SE-shSec23a cells with FAM134B interference. Autolysosomes indicated by arrowheads, bar = 5 μm. i Mean number of detectable autolysosomes in each tumor cell, counted on transmission electron microscopy images. *P < 0.05, **P < 0.01, ***P < 0.001