Fig. 1
From: Sec23a inhibits the self-renewal of melanoma cancer stem cells via inactivation of ER-phagy
Generation and characterization of M14 derived cancer stem cell line. a The morphology of M14-Parental and M14-SE cells, bar = 120 µm. b Analysis of mRNA expression of Aldh1, CD133, Nanog, Oct4 and Sox2. The GAPDH expression was used as a reference control. c–e. Clonogenic spheroid formation rate, morphology and average spheroid diameter of M14-Parental and M14-SE cells in the single-cell cloning assay. f Serial spheroid formation assay of M14-Parental and M14-SE cells, 1st,2nd and 3rd means the first, second and the third round of the spheroid assay. bar = 200 µm. g Tumors resected from nude mice inoculated with M14-Parental and M14-SE cells, respectively, bar = 1 cm. h and i. Tumor volume and weight, **P < 0.01, ***P < 0.001