Fig. 7

Effects of UHMK1 on coilin phosphorylation and 5-FU sensitivity in SW480 cells. a Morphology changes of CBs in 1 μg/ml 5-FU and removal. b Western blots for coilin phosphorylation and UHMK1 expression. c Co-immunoprecipitation assay for coilin serine phosphorylation in UHMK1 knockdown cells. d Coilin phosphorylation in UHMK1 and its mutant transfected SW480 cells. e CCK8 assays for cell growth of UHMK1 knockdown cells. f Survival of UHMK1 knockdown SW480 cells following 5-FU treatments (0, 1, 5, 10, 20, 40, 60 and 80 μg/ml) for 48 h. g Cell growth of transfected SW480 by CCK8 assays. h Dose-dependent survival from 5-FU treatments (0, 1, 5, 10, 20, 40, 60 and 80 μg/ml) for 48 h of transfected SW480 cells. Data were presented as the mean ± SD, n = 6. * p < 0.05, ** p < 0.01