Fig. 3

RNA-seq analyses for differentially expressed (DE) genes and alternative splicing events comparing HCT-8/FU cells and HCT-8 cells in both normal culture and CM treatment from HCT-8/FU cultures. a Venn diagram illustrating the total and shared numbers of DE genes. b Venn diagram summarizing the number of differences in identified splicing isoforms (SIs). c Enrichment from Gene Ontology for biological processes on DE genes and differential SIs. d Venn diagram on gene sets of kinase activity (GO: 0016301), RNA binding (GO: 0003723) and nucleus (GO: 0005634). e Volcano plots and identification of DE genes between HCT-8/FU and HCT-8 cells. f Volcano plots of DE genes between HCT-8 treated with CM of HCT-8/FU and CM of HCT-8. g Protein (left) and mRNA (right) expression of UHMK1 in HCT-8 and HCT-8/FU cells. h UHMK1 expression in HCT-8 cells treated with CM from HCT-8 and HCT-8/FU