Fig. 1

Morphology of Cajal bodies (CB) and coilin phosphorylation in HCT-8 and 5-FU resistant HCT-8/FU cells. a Immunofluorescent of coilin in cells treated with 1 μg/ml 5-FU for 24 h. b CB morphology in HCT-8 cells following treatments of chemotherapeutic reagents of 5-FU (1 μg/ml), apatinib (20 µM), raltitrexed (2.5 µM), artemisinin (100 µM) and cisplatin (10 μg/ml) for 24 h. c Survival of HCT-8 and HCT-8/FU cells treated with 5-FU of increasing doses (0, 1, 10, 20, 40 μg/ml) for 48 h by CCK8 assays. d Percentage in counts of various CB morphological categories in HCT-8 and HCT-8/FU cells. e Western blots for coilin expression in HCT-8 and HCT-8/FU cells. f Determination of coilin phosphorylation in HCT-8 and HCT-8/FU cells