Skip to main content
Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Heterocellular N-cadherin junctions enable nontransformed cells to inhibit the growth of adjacent transformed cells

Fig. 5

Nontransformed cells decrease PDPN and increase p120-catenin expression in adjacent transformed cells. a Src transformed (MEC-Src) or HSC-2 cells, and nontransformed cells with (MEC) or without (MEC-CdhKo) N-Cdh expression were above or beneath a porous membrane at a 1:3 ratio, respectively. This membrane permits junctional communication but prevents actual mixing of the cell populations. b Each population of transformed and nontransformed cells in this layered culture system were separately harvested 24 h after plating, and analyzed for PDPN, p120-catenin, and GAPDH expression by Western blot analysis as indicated. c Western blot results were quantitated and shown as percent of Src transformed (MEC-Src) cells plated over MEC-Src cells or percent of HSC-2 cells plated over HSC-2 cells (mean + SEM, n = 3, n = 5). Single asterisk, double asterisks, and ns indicate differences with p < 0.05, p < 0.01, or p > 0.05, respectively, by t-test as indicated

Back to article page