Fig. 1

Cadherins are required for contact normalization. a Src transformed mouse embryonic cells (MEC-Src), human OSCCs (HSC-2), and Src transformed cells transfected with PDPN (MEC-SrcPdpn) were labeled with DiI (red). These labeled transformed cells were then plated at a 1:300 ratio with unlabeled transformed cells, nontransformed wild type mouse embryonic cells (MEC), or cadherin knockout mouse embryonic cells (MEC-CdhKo). Cultures were grown for 7 days and visualized by phase contrast and fluorescence microscopy as indicated (scale bar = 500 µm). b The number of transformed (red) cells in a 425 × 570 µm field were counted and shown as percent of transformed cells plated alone (mean + SEM, n = 4). Double, triple, and quadruple asterisks indicate differences with p < 0.01, p < 0.001, and p < 0.0001, respectively, by t-test as indicated