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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Infrapatellar fat pad adipose-derived stem cells co-cultured with articular chondrocytes from osteoarthritis patients exhibit increased chondrogenic gene expression

Fig. 2

Histology demonstrating the trilineage potential of IFPD ASCs. A 40× and B 100× magnifications of an Alcian Blue stained chondrogenic pellet section indicating the presence of glycosaminoglycans found in cartilage ECM from ASCs cultured in chondrogenic medium. ASCs were unable to form a pellet in control using non-differentiating medium from the experiment protocol, hence no control staining is provided. C 100× magnification of Alizarin Red S stained calcium deposits demonstrating osteogenesis in ASCs cultured in osteogenic medium. D 200× magnification of Alizarin Red S applied to ASCs cultured in control medium, no calcium deposits demonstrated. E 100× magnification of Oil Red O stained lipid vesicles demonstrating adipogenesis in ASCs cultured in adipogenic medium. F 200× magnification of Oil Red O stained ASCs cultured in control medium, no lipids vesicles demonstrated

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