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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Na+/Ca2+ exchanger isoform 1 takes part to the Ca2+-related prosurvival pathway of SOD1 in primary motor neurons exposed to beta-methylamino-l-alanine

Fig. 3

Effect of SOD1 and ApoSOD1 on NCX1-mediated [Ca2+]i influx and [Na+]i in rat primary motor neurons. A Co-localization between plasmalemmal NCX1 and SOD1 in a representative rat primary motor neuron at two different magnifications (see arrows). B Quantification of SOD1 and ApoSOD1-induced [Na+]i increase in SBFI-loaded motor neurons (see representative images on the top). Data are quantified as Δ% of increase in 35 cells for each group. All experiments were repeated at least three times; *p < 0.05 versus internal control (basal values of [Na+]i). C Superimposed representative traces of the effect on [Ca2+]i of SOD1 (400 ng/ml) in Na+-free solution and ApoSOD1 (400 ng/ml) in Na+-free solution both perfused on siControl neurons or siRNA-treated neurons against NCX1 (10 nmol/L for 48 h) loaded with Fura-2 (see representative images on the top). D Quantification of the effect of C reported as Δ% of increase in 40 cells for each group. All the experiments were repeated at least three times; *p < 0.001 versus SOD1 or ApoSOD1 alone (in the presence of external Na+); **p < 0.05 versus “SOD1 + Na+-free” or “ApoSOD1 + Na+-free”

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