Fig. 1
Effect of the pharmacological inhibitors of P2X7, cADP-ribose and NCX on SOD1- and ApoSOD1-induced [Ca2+]i increase in rat primary motor neurons. A Superimposed representative traces of the effect on [Ca2+]i of SOD1 alone (400 ng/ml) or in combination with CB-DMB (1 µM) in Fura-2-loaded primary motor neurons. B Quantification of the effect of SOD1 (400 ng/ml) alone (n = 30 cells) and in the presence of CB-DMB (1 µM) (n = 25 cells), the specific antagonist of P2X7 receptor, A430879 (1 μM) (n = 35 cells) or the cell permeant antagonist of cADP-ribose, 8-bromo-cADPR (10 μM) (n = 28 cells). Primary motor neurons were pre-incubated with 8-bromo-cADPR, A430879 or CB-DMB for 10 min before [Ca2+]i recordings. All the experiments were repeated at least three times; *p < 0.05 versus internal control (basal values of [Ca2+]i), **p < 0.05 versus internal control and SOD1 alone. C Superimposed representative traces of the effect on [Ca2+]i of ApoSOD1 alone (400 ng/ml) or in combination with CB-DMB (1 µM) in Fura-2-loaded primary motor neurons. D Quantification of the effect of ApoSOD1 (400 ng/ml) alone (n = 29 cells) and in the presence of CB-DMB (1 µM) (n = 30 cells), the specific antagonist of P2X7 receptor, A430879 (1 μM) (n = 35 cells) or the cell permeant antagonist of cADP-ribose, 8-bromo-cADPR (10 μM) (n = 30 cells). Primary motor neurons were pre-incubated with 8-bromo-cADPR, A430879 or CB-DMB for 10 min before [Ca2+]i recordings. All the experiments were repeated at least three times on different cultures; *p < 0.001 versus internal control (basal values of [Ca2+]i), **p < 0.05 versus internal control and ApoSOD1 alone