Fig. 7

IGBP1 is essential for PP2A-dependent JNK activation and apoptosis upon TPA stimulation. a PC3 and αT3-1 cells were treated with scramble (SCR) or IGBP1 SiRNA for 48 h. Cells were then grown to 70% confluency, serum starved (0.1% serum for 16 h) and then treated with or without TPA (250 nM, 30 min). Cells were harvested in RIPA buffer and the cell extracts were separated and immunoblotted with the indicated antibodies. The bar-graphs represent average and standard errors of three distinct experiments. b Cells were treated as in A, except that the TPA (250 nM) was added for 48 h. Then the cells were harvested in RIPA buffer and the cell extracts were separated and immunoblotted with the indicated antibodies. The bar-graphs represent average and standard errors of two distinct experiments. c Cells were treated as in B followed by fixation with 4% PFA and apoptotic TUNEL assay. The bar-graphs represent average and standard errors of three distinct experiments NT-non-treated. In all cases: * p < 0.01