Fig. 5
From: GqPCR-stimulated dephosphorylation of AKT is induced by an IGBP1-mediated PP2A switch
CoIP of PP2A components with PI3K and AKT. a CoIP of the PP2A components with PI3K. Serum starved αT3-1 and PC3 cells were either stimulated with TPA (250 nM, 30 min; + , Con) or left untreated as control (-) and then harvested. Then, anti PI3K Ab or non-relevant Ab (anti histone H1, Con) were used for IP, and the CoIPed proteins, as indicated, were detected by the relevant Abs. b Quantification of the results in A. The bar-graph represent means ± standard errors of three experiments for the indicated proteins.* p < 0.01, ** p < 0.05. c CoIP of the PP2A components with AKT. Serum starved αT3-1 and PC3 cells were either stimulated with TPA (250 nM, 30 min; + , Con) or left untreated, and then harvested. Then, anti AKT Ab or non-relevant Ab (anti KRas, Con) were used for IP, and the CoIPed proteins were detected by the indicated Abs. d Quantification of the results in C. The bar-graphs represent means ± standard errors of three experiments of the indicated proteins.* p < 0.01, ** p < 0.05