Table 1 Primer sequence and PCR condition
Gene name | Primer sequences | Tm (°C) | Cycles | Length (bp) | Accession number |
|---|---|---|---|---|---|
IL-1α | F: 5′-AATGACGCCCTCAATCAAAG-3′ | 54 | 35 | 206 | NM-000575 |
R: 5′-TGGGTATCTCAGGCATCTCC-3′ | |||||
IL-1 RI | F: 5′-CGGCAGGAATGTGACAATCG-3′ | 56 | 35 | 178 | AH008153.2 |
R: 5′-TCTCACCCCTACCTAGTCCC-3′ | |||||
CXCL12 | F: 5′-TTCCATTTGCAAGGGAAAAG-3′ | 56 | 35 | 236 | NM-000609 |
R: 5′-ACACACAGCCAGTCAACGAG-3′ | |||||
CXCR4 | F: 5′-GAAGCTGTTGGCTGAAAAGG-3′ | 56 | 35 | 345 | NM-003467 |
R: 5′-GAGTCGATGCTGATCCCAAT-3′ |