Fig. 8

Effect of PLX8394 “paradox breaker” on HDF. HDF were stimulated with 2 µM of PLX8394, 5 nM of trametinib (TR), or the combination. A Flow cytometric analysis of the proliferative state after 18 h of inhibition. Top: quantification of the percentage of proliferative cells (Ki67 positive) for four independent experiments (n = 4). Bottom: representative histograms showing the frequency distribution of Ki67 levels in the indicated condition. B Flow cytometric analysis of the cell viability after 66 h of inhibition. Top: quantification of the percentage of viable cells (Annexin V–PI negative) for four independent experiments (n = 4). Bottom: Representative scatter plots showing the viable (lower left quadrant) and apoptotic/necrotic cells. All bar plots show the mean ± SEM of the experiments. **p < 0.01, ****p < 0.0001