Fig. 6
From: Cytoplasmic eIF6 promotes OSCC malignant behavior through AKT pathway
eIF6 physically interacts with AKT. (A) Immunoprecipitation assay between endogenous eIF6 and AKT. (B) The protein levels of eIF6 and AKT were detected in cells treated with MG132 (10 μM). (C) The protein levels of eIF6 and AKT were detected in cells treated with CHX (20 μM). (D) Cells over-expressing eIF6 were treated with or without MG132, and the eIF6 and AKT protein levels were assessed by Western blotting. (E) CHX was utilized in cells transformed with eIF6 or NC plasmids, and the protein expression levels of eIF6 and AKT were displayed. (F) Co-IP experiment showed MG132 promoted the binding extent between eIF6 and AKT. The cells in eIF6 over-expression group were treated with or without MG132. Cell lysates were prepared and subjected to immunoprecipitation with anti-eIF6 antibody. The level of AKT was detected by western blotting analysis. (G) Graphical abstract demonstrated the regulation of eIF6/AKT axis in the cytoplasm