Fig. 5
From: Cytoplasmic eIF6 promotes OSCC malignant behavior through AKT pathway
eIF6 participates in the activation of AKT in the cytoplasm. (A) Confocal microscopy showed that eIF6 was mainly expressed in the cytoplasm upon eIF6 amplification compared with control. (eIF6, red; DAPI depicts nuclei, blue). (B,C) Increased immunostaining of AKT (B) and p-AKT (C) in the cytoplasm was shown upon ectopic expression of eIF6. (D) eIF6 augmentation resulted in the upregulation of cytoplasmic expression of eIF6, AKT and p-AKT, while nucleus expression of eIF6, AKT and p-AKT was reduced after transfection. (E) Cell-cycle related proteins were further analyzed in the subcellular fractions. All error bar values represent the SD. Scale bar, 20 μm. *P < 0.05, **P < 0.01, ***P < 0.001