Fig. 6

CELsignia test is a more sensitive and rapid test for detecting drug efficacy compared to the biological correlates. HCC1954 cells were seeded in collagen-fibronectin (CF) coated culture plates and treated 6 h later with tepotinib (250 nM) and neratinib (250 nM), either singly or in combination, as indicated. Cells were harvested and then analyzed by flow cytometry for the markers shown following a period of 16 h (a) or 48 h (b) of drug treatment. The data indicate relative insensitivity of flow cytometry markers to deteriorating cell health until at least 48 h after drug application. At 48 h, annexin v expression has increased by nearly twofold for cells treated with combined pan-HER and cMET targeted drugs