Fig. 6
PMV-delivered CTGF improved the function of VEC by activating ERK1/2. A The expression and the phosphorylation level of ERK1/2, p38 MAPK, and JNK in LPS-stimulated VECs treated by modified PMVs (n = 3). B CCK8 proliferation of LPS-stimulated VECs treated by modified PMVs with or without ERK1/2 agonist (n = 6). C Scratch experiment of LPS-stimulated VECs treated by modified PMVs with or without ERK1/2 agonist, the representative images are shown (n = 5, bar = 100 μm). D Tube formation of LPS-stimulated VECs treated by modified PMVs with or without ERK1/2 agonist, the representative images are shown (n = 5, bar = 50 μm). (E) Statistical analysis of the tube length in D (n = 5). Ctl: normal group; LPS: LPS group; PMV: PMV-treated LPS group; PMVCTGF−down: CTGF-downregulated PMV + LPS group. ERK-ago: ERK1/2 agonist Senkyunolide I. ***P < 0.001, **P < 0.01