Table 1 Primers in real-time PCR
From: STING protects against cardiac dysfunction and remodelling by blocking autophagy
Gene | Species | Forward primer | Reverse primer |
|---|---|---|---|
IL-1β | Mouse | AATGAAGGAACGGAGGAGCC | CTCCAGCCAAGCTTCCTTGT |
IL-6 | Mouse | GTTGCCTTCTTGGGACTGATG | ATACTGGTCTGTTGTGGGTGGT |
MCP-1 | Mouse | TGGCTCAGCCAGATGCAGT | CCAGCCTACTCATTGGGATCA |
HMGB1 | Mouse | CCGGCAAGTTTGCACAAAGA | TTGGGAGGGCGGAGAATCAA |
GAPDH | Mouse | ACTCCACTCACGGCAAATTC | TCTCCATGGTGGTGAAGACA |
ANP | Mouse | ACCTGCTAGACCACCTGGAG- | CCTTGGCTGTTATCTTCGGTACCGG |
Collagen1 | Mouse | AGGCT TCAGTGGTT TGGATG | CACCAACAGCACCATCGTTA |
Collagen3 | Mouse | CCCAACCCAGAGATCCCATT | GAAGCACAGGAGCAGGTGTAGA |
Fibronectin | Mouse | CGGTGGCTGTCAGTCAGA | TCCCACTGCTGATTTATC |
CTGF | Mouse | TGTGTGATGAGCCCAAGGAC | AGTTGGCTCGCATCATAGTTG |
STING | Mouse | ATCTATGCTAGTCGTAGTTTA | CGTAGTGCTAGTGATTAGTC |
ANP | Rat | ATGGGCTCCTTCTCCATCAC | TCTTCGGTACCGGAAGCTG |
BNP | Rat | TTCCTTAATCTGTCGCCGCTGG | CAGCAGCTTCTGCATCGTGGAT |
Collagen1 | Rat | GAGAGAGCATGACCGATGGATT | TGGACATTAGGCGCAGGAA |
Collagen3 | Rat | CAGACGGGAGTTTCTCCTCGGA | GACCAGGAGGACCAGCAACTCC' |
GAPDH | Rat | GACATGCCGCCTGGAGAAAC | AGCCCAGGATGCCCTTTAGT |