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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Estradiol increases risk of topoisomerase IIβ-mediated DNA strand breaks to initiate Xp11.2 translocation renal cell carcinoma

Fig. 5

Combined effect of TOP2β and ERα in mediating DNA breaks. A γH2AX focis detected in HK-2 cells under 10 nM E2 treatment for 48 h or a concentration gradient of etoposide (0, 1, 2, 5, 10, 20, 50, 100 μM) for 1 h. B Quantification of the γH2AX focis in cells as in panel A. C and D γH2AX focis detected in HK-2 cells transfected with negative control siRNA (NC), siRNA against TOP2A (siTOP2A), or siRNA against TOP2B (siTOP2B) under 0.1% DMSO (solvent-only control), 10 nM E2, 10 μM etoposide or a combination of the two drugs and their quantification. E and F γH2AX focis detected in HK-2 cells transfected with negative control shRNA (NC), shRNA against ESR1 (shESR1), or shRNA against ESR2 (shESR2) under 0.1% DMSO (solvent-only control), 10 nM E2, 10 μM etoposide or a combination of the two drugs and their quantification. G Western blot of ERα co-immunoprecipitation (Co-IP) detected with anti-Top2β antibodies. Error bars indicate 95% confidence intervals (***p < 0.001)

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