Fig. 4

E2 induced-TFE3 breaks through ERα-dependent pathway. A γH2AX focis detected in HK-2 cells transfected with negative control shRNA (NC), shRNA against ESR1 (shESR1), or shRNA against ESR2 (shESR2) under 0.1% DMSO (solvent-only control) or 10 nM E2 treatment for 48 h. B Quantification of the γH2AX focis in E2-treated cells as in panel A. C Quantification of the MNs in E2-treated cells as in panel A. D Quantification of the TFE3 break-apart signals in HK-2 cells transfected with NC or shESR1 under 0.1% DMSO (solvent-only control) or 10 nM E2 treatment for 48 h. E and F Statistics of TOP2β ChIP-qPCR results performed using the primers near the translocation sites in HK-2 cells under 0.1% DMSO (solvent-only control) or 10 nM E2 treatment for 48 h. ChIP-qPCR was normalized to Input DNA, experiments were repeated three times and data shown are means ± SD. Error bars indicate 95% confidence intervals (**p < 0.01; ***p < 0.001)