Fig. 3

Etoposide-induced TOP2-mediated TFE3 breaks in Xp11.2 translocation renal cell carcinoma (tRCC) translocation sites. A Quantification of the TFE3 break-apart signals in HK-2 cells under 0.1% DMSO (solvent-only control) or 100 μM etoposide treatment for 1 h. B Quantification of the TFE3 break-apart signals in HK-2 cells transfected with NC or siTOP2B under 0.1% DMSO (solvent-only control) or 100 μM etoposide treatment for 1 h. C Ideograms of the translocation fragments of two Xp11.2 tRCC cell lines, UOK109 and UOK120. D and E Statistics of TOP2β chromatin immunoprecipitation (ChIP)-qPCR results performed using the primers near the translocation sites as in panel c in HK-2 cells under 0.1% DMSO (solvent-only control) or 100 μM etoposide treatment for 1 h. F and G Statistics of CTCF ChIP-qPCR results performed using the same primers as in panel D and E in HK-2 cells. ChIP-qPCR was normalized to Input DNA, experiments were repeated three times and data shown are means ± SD. Error bars indicate 95% confidence intervals (*p < 0.05; **p < 0.01; ***p < 0.001)