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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Estradiol increases risk of topoisomerase IIβ-mediated DNA strand breaks to initiate Xp11.2 translocation renal cell carcinoma

Fig. 2

Etoposide-induced topoisomerase II (TOP2)-mediated DNA breaks and chromosomal breaks. A γH2AX focis detected in HK-2 cells under 0.1% DMSO (solvent-only control) or 100 μM etoposide treatment for 1 h. B Quantification of the γH2AX focis in etoposide-treated cells as in panel A. C γH2AX focis detected in HK-2 cells transfected with negative control siRNA (NC), siRNA against TOP2A (siTOP2A), or siRNA against TOP2B (siTOP2B) under 0.1% DMSO (solvent-only control) or 100 μM etoposide treatment for 1 h. D Quantification of the γH2AX focis in etoposide-treated cells as in panel C. E Quantification of the micronucleis (MNs) in etoposide-treated cells as in panel C. Error bars indicate 95% confidence intervals (***p < 0.001)

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