Fig. 2
From: Identification of Desmoglein-2 as a novel target of Helicobacter pylori HtrA in epithelial cells
H. pylori-mediated Desmoglein-2 during infection of gastric epithelial cells is dependent on HtrA. Gastric epithelial cells were infected with H. pylori strains P12 wt, N6 wt and N6 ΔhtrA for 24 h or left uninfected (mock). A Supernatants of infected NCI-N87 cells were analyzed by Western blotting for soluble N-terminal hDsg2 (hDsg2NTF) and hCdh1 (hCdh1NTF) cleavage fragments using antibodies recognizing the extracellular domains of hDsg2 and hCdh1. B The amount of soluble hDsg2NTF in supernatants was quantified and data are presented as mean values of hDsg2NTF ± SD (n = 3). Asterisks indicate statistically significant differences (**p < 0.01; ns, not significant). C Whole cell lysates of infected NCI-N87 cells (left panel) and infected MKN28 cells (right panel) were analyzed by Western blotting for full length hDsg2 (hDsg2FL) using an antibody recognizing the intracellular domains of hDsg2. GAPDH, CagA and HpHtrA were detected as controls