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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Beclin1 controls caspase-4 inflammsome activation and pyroptosis in mouse myocardial reperfusion-induced microvascular injury

Fig. 5

Enhanced beclin1 expression results in inhibition of caspase-4 activation and pyroptosis in mice. A Becn1 overexpression effect was observed. GSDMD expression in WT and BECN1-Tg heart was assessed by western blot analysis. B Densitometric quantification of Beclin1, LC3I/LC3II, p62, caspase-4, IL-1β and GSDMD in the heart and Becn1 and GSDMD in the spleen and liver. Data are expressed as mean ± SD (n = 3). *P < 0.05 vs. WT group, **P < 0.05 vs. WT-I/R group. C Beclin1, LC3, p62, caspase-4, IL-1β and GSDMD expression in WT and BECN1-Tg heart under Sham or 45 min ischemia/6 h of reperfusion was assessed by western blot analysis. D GSDMD expression in WT and BECN1-Tg spleen and liver after 45 min myocardial ischemia/6 h of reperfusion as assessed by western blot analysis. E, F Immunofluorescence staining and quantification of GSDMD in WT and BECN1-Tg heart in Sham or 45 min ischemia/6 h of reperfusion. Data are expressed as mean ± SD (n = 3). Scar bar = 20 μm. *P < 0.05 vs. WT-I/R group. G Expression of GSDMD in WT and BECN1-Tg spleen and liver after 45 min myocardial ischemia/6 h of reperfusion as determined by immunofluorescence. H Quantification showed a remarkably decreased expression of GSDMD in BECN1-Tg group. Data are expressed as mean ± SD (n = 3). Scar bar = 20 μm. *P < 0.05 vs. WT group

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