Fig. 4From: Beclin1 controls caspase-4 inflammsome activation and pyroptosis in mouse myocardial reperfusion-induced microvascular injuryCaspase-4 inflammasome and pyroptosis-associated proteins are activated in heart and CMECs after MI/R. A–D Representative western blotting bands and densitometric quantification of GSDMD, caspase-4 and IL-1β in heart at different myocardial ischemia/reperfusion (MI/R) time points. Data are expressed as mean ± SD (n = 3). *P < 0.05 vs. Sham group. E, F Representative western blotting bands and densitometric quantification of caspase-4, IL-1β and GSDMD were assessed in CMECs at different hypoxia/reoxygenation (H/R) time points. Data are expressed as mean ± SD (n = 3). #P < 0.05 vs. control group. G Immunofluorescence staining for GSDMD in CMECs at 2 h of hypoxia/2 h of re-oxygenation. Scar bar = 20 μmBack to article page