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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: EGF stimulates human trophoblast cell invasion by downregulating ID3-mediated KISS1 expression

Fig. 4

Downregulation of ID3 mediates the EGF-suppressed KISS1 expression. a, b HTR-8/SVneo cells were treated with 50 ng/mL EGF for 1, 3, and 6 h. The ID3 mRNA levels (a) and protein levels (b) were examined by RT-qPCR and western blot, respectively. c, d HTR-8/SVneo cells were pretreated with vehicle control (DMSO) or 5 µM AG1478 for 1 h, and then treated with 50 ng/mL EGF for 3 h. The ID3 mRNA levels (c) and protein levels (d) were examined by RT-qPCR and western blot, respectively. e, f HTR-8/SVneo cells were pretreated with vehicle control (DMSO) or 5 µM LY294002 for 1 h, and then treated with 50 ng/mL EGF for 3 h. The ID3 mRNA levels (e) and protein levels (f) were examined by RT-qPCR and western blot, respectively. g, h HTR-8/SVneo cells were transfected with 1 µg control vector (pCMV) or vector containing human ID3 cDNA (pCMV-ID3) for 48 h. After transfections, cells were treated with 50 ng/mL EGF for 24 h. The mRNA (g) and protein (h) levels of KISS1 and ID3 were examined by RT-qPCR and western blot, respectively. Numbers under the western blots represent the densitometry quantifications. The results are expressed as the mean ± SEM of at least three independent experiments. Values without a common letter are significantly different (p < 0.05)

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