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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: Zinc-mediated activation of CREB pathway in proliferation of pulmonary artery smooth muscle cells in pulmonary hypertension

Fig. 6

Role of zinc in regulation of CREB-mediated transcription and cell proliferation. a Representative zinc probe fluorescence images in PASMCs isolated from control and MCT-treated rats. The intracellular labile zinc levels in PASMCs were stained by fluozin-3. b Summarizing the relative intensities of fluorescence in PASMCs. n = 4. c The effect of zinc on CRE-luciferase reporter activity in PH-PASMCs. PASMCs were infected with pGL4.20-TH reporter, an adenoviral vector expressing a CRE-luciferase. n = 4. d The effect of zinc on PASMCs proliferation determined by MTT. Serum starved cells were pretreated with TPEN (10 μM) for 24 h. n = 6. TPEN, N,N,N′,N′-Tetrakis (2-pyridylmethyl) ethylenediamine; Ctr, control; MCT, MCT treatment for 4 weeks. *p < 0.05 vs. Ctr, #p < 0.05 vs. MCT. e Elevated expression of ZIP12 protein in rat lung homogenate. n = 8. f Elevated expression of ZIP12 protein in isolated PH-PASMCs. n = 4. Ctr, control; MCT, MCT treatment for 4 weeks. *p < 0.05 vs. Ctr

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