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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Zinc-mediated activation of CREB pathway in proliferation of pulmonary artery smooth muscle cells in pulmonary hypertension

Fig. 5

The effect of CREB-mediated transcriptional activity on PASMCs proliferation. a, d The elevated cell proliferation in PH-PASMCs. The PASMCs were isolated from control and MCT-treated rats. n = 6, 4. b, e The inhibitory effect of FK506 on cell proliferation. Cells were starved with serum-free DMEM/F12 for 24 h, and then treated with FK506 (1 nM) for 24 h. n = 6, 5. c, f The inhibitory effect of CREM on cell proliferation. Cells were starved with serum-free DMEM/F12 for 24 h and transfected with Ad-CREM and Ad-GFP for 24 h. n = 6, 5. Cell proliferation was determined by MTT assay (a-c) and measurement of PCNA expression (d–f). Ad-CREM, ICER-expressing adenovirus vector; Ad-GFP, green fluorescent protein-empty adenovirus vector. Ctr, control; MCT, MCT treatment for 4 weeks. *p < 0.05 vs. Ctr, #p < 0.05 vs MCT

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Cell Communication and Signaling

ISSN: 1478-811X